EMBL Course: Advanced Fluorescence Imaging Techniques

This practical course will cover advanced light microscopy techniques and participants will learn how to derive qualitative and quantitative insights on molecular mechanisms in cells and developing organisms. Invited guest researchers together with experts from the EMBL and microscopy professionals will foster an intense information flow with a balance of lectures and practical workshops. The focus of the course will be on the use of fluorescence microscopy to obtain quantitative information about protein dynamics in living samples.

Topics: Time-lapse microscopy, Confocal microscopy, Total internal reflection fluorescence microscopy (TIRFM), Super resolution microscopy (PALM, STORM), Expansion microscopy, Fluorescence resonance energy transfer (FRET), Fluorescence recovery after photobleaching (FRAP), Photoconversion, Photoactivation