During the last four decades, a number of studies have revealed that Wnt signaling plays crucial roles in embryonic development, tissue morphogenesis and regeneration, and many other physiological and pathological processes. Therefore, the knowledge about this signaling is crucial not only for the understanding of multiple aspects of physiology in all life courses but for that of pathology in human diseases. Since Wnt research covers various fields of the life science, this meeting is aiming to cover “molecular and cellular biology of the signaling”, “structural biology”, and “stem cell biology”, as well as “clinical application”. An important objective of this EMBO Workshop on Wnt 2022 is to bring together researchers from different fields for exchanging cutting-edge information that advance this research field.