In spite of their omnipresence and importance, a number of questions about knots remain elusive. Addressing them solicits techniques from a range of mathematical disciplines at the interface of algebra, analysis, geometry, modeling, and low-dimensional topology. Some of the most exciting recent avenues of research include optimizing geometry, quantum knot invariants, and applications in material sciences, physics, and molecular biology. This workshop emphasizes bridging the gap between theoretical, computational, and experimental approaches in knot theory and its applications, including artificial intelligence.