EMBO Practical Course — Characterisation of post-translational modifications in cellular signalling

This EMBO practical course aims to provide a theoretical and practical overview of state-of-the-art techniques for the characterization of various PTMs, such as phosphorylation, acetylation, cysteine PTMs and glycosylation, and their cross-talking in controlling dynamic cellular mechanisms. The laboratory part will focus on a case study of EGF stimulated SILAC labeled HeLa cells. You will be introduced to enrichment, identification and quantitation of the PTM peptides and to mass spectrometry analysis as well as data interpretation and bioinformatics. This will provide you with knowledge needed to perform quantitative large-scale PTMomics from small amounts of sample material yourself. You will be taught in methods developed in the laboratories of the speakers and they will provide you with their own tips and tricks.