This two day annual confocal course utilises many different sample types and fluorescent probes (DNA stains, classic antibody labels and fluorescent proteins) which are chosen to best demonstrate particular problems and techniques. Focus is always on the techniques they enable and the problems they generate, which will be applicable to any sample types. Day 1 takes participants through the basic principles of confocal microscopy and then shows them how to configure and image multicolour, multidimensional samples using a confocal microscope. Day 2 builds on the experience of day 1 and demonstrates FRAP and spectral profiling.