This meeting will be the fifth iteration of this highly successful Journal Meeting, which has been running every two years since 2014 (including virtually in 2020). Over the past four events, we have brought together a diverse group of researchers (both in terms of topic and demographics) united in their desire to better understand human development, and to do so using human cells and tissues. Since the first meeting in 2014, we have witnessed huge advances in the field, driven largely by technological advances such as organoid culture and single cell sequencing, and have seen significant expansion in the range of questions that can be now addressed using human systems. It is therefore more important than ever to provide a forum for researchers to come together and discuss the latest advances, and remaining challenges, in the field.