The biogenesis and maintenance of cellular organelles requires sophisticated biological machineries to target proteins to, and translocate them across, their corresponding membranes. Research to date has provided fantastic insights into the molecular mechanisms of an impressive variety of prokaryotic and eukaryotic transport machineries. However, key questions regarding the molecular structures, regulation, and dynamics of these protein transport complexes remain unsolved. Our workshop will focus on the up-to-date advances in our understanding of protein transport, relying heavily on cutting edge techniques. This EMBO Workshop will provide a platform for young and established researchers to enjoy excellent talks, contribute to vivid discussions, and enjoy superb networking opportunities.