The ability to grow tissues from stem cells in 3D culture to generate so-called organoids has revolutionised the study of tissue biology, especially human biology. This exciting technology has great potential for drug discovery and regenerative medicine. Because organoids model a variety of tissues with remarkably high fidelity, they allow for new discoveries in human development, homeostasis, regeneration, and disease. This symposium will bring together the leading researchers in this field, representing the premier conference for this research community, and providing a much-anticipated program that demonstrates how this field is truly pushing the boundaries for our understanding of tissue biology. This, the fifth edition of this established meeting will again bring together researchers from different fields to enhance our understanding of how organoids can be formed and maintained and their biomedical applications, from how they can be exploited to study disease to eventually use them to regenerate and replace human tissues. An aim for the 2025 edition of the symposium is the incorporation of more complementary technologies, such as multiomics, engineered embryo models and bioengineering as well as increased representation of the topic of cancer.