This EMBO Workshop will bring together experts in diverse domains of bacterial biology for a four-day meeting focused on discussing frontier aspects of bacterial morphogenesis, survival and virulence. Based on the previous edition of the meeting in 2016 (Thiruvananthapuram, Kerala) and BMSV 2019 (Cape Town, South Africa), this EMBO Workshop will comprehensively cover current technologies and ideas in bacterial research including themes of cell division, signaling, genome biology, stress response, morphogenesis and virulence.