Flow Cytometry is one of the most powerful single cell analysis methods used throughout the life sciences and medicine. By utilising Flow Cytometry we can identify and quantify specific cell populations from heterogeneous samples, analyse the cell cycle or proliferation state or simply detect the expression of fluorescent reporters. In line with the suggestions from our course in 2019, we will significantly enhance the number of analysis sessions together with our partners from FlowJo. We will cover the theory of Flow Cytometry and corroborate this information intensively with the practical issues of the most frequent assays as part of our practical course curriculum.